Plasmid

Part:BBa_J100169

Designed by: Kathryn Smith   Group: Campbell M Lab   (2014-07-10)


P5 promoter + M6''-R6 ammeline riboswitch + RFP

This riboswitch sequence was modeled off of the original riboswitch sequence found in part J119335. It was designed to be a more effective riboswitch by inserting and changing nucleotides in the riboswitch sequence. It has two mutations from the design in the RFP (A to G at 432 a change from tyrosine to cysteine, and A to G at 673 which causes no amino acid change.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 742
    Illegal AgeI site found at 854
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None